Another day, another squid illustration! This one is for my colleague, Bas Leemburg, for a review on insights into the microbial response to the outside environment through cryo-ET. The goal of the illustration was to showcase and summarize some of the new techniques developed in the field of cryo-EM over the last 2 to 3 years, mainly for the large volume workflow. Because this image is similar to the Large volume cryoEM workflow I made for Dr. Katrina Gundlach, I wanted to at least create a new squid!
In this image I highlight the biology of the symbiosis between the squid's light organ and the bacterium Vibrio fisheri (A), the serial lift-out technique (B,C), the tilt series collected through cryoEM (D), the reconstruction of this tiltseries and the segmentation of the structures (E), and the high resolution reconstructions of the ribosomes in Vibrio fisheri (F). This work is part of the publication "Beyond resolution: cryo-electron tomography of microbial nanomachines in native host context" in Current opinion in structural biology (1).
Except where otherwise noted this work is protected under https://creativecommons.org/licenses/by-nc-nd/4.0/
References
1. Bas Leemburg, Mohamad Harastani, Armel Bezault, Ariane Briegel, Beyond resolution: Cryo-electron tomography of microbial nanomachines in native host context, Current Opinion in Structural Biology, Volume 98, 2026, 103280, ISSN 0959-440X, https://doi.org/10.1016/j.sbi.2026.103280
